How long in vitro transcripts?
mat at biobase.dk
Tue Mar 17 11:41:29 EST 1998
Does anyone have experience in producing in vitro transcripts 4-5kb
long? We are considering this for a cDNA almost 5000 bases long, with
rather long 5' and 3' untranslated regions (the coding region is "only"
3000 bases). We need to transcribe the whole length in order to look for
the accessible sites for antisense oligos. I would greatly appreciate
any suggestions or comments!
Department of Medical Physiology The Panum Institute
University of Copenhagen Blegdamsvej 3C
Tel. +45-35327510 Fax +45-35327537 2200 Copenhagen N
E-mail mat at biobase.dk Denmark
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