EDTA and MgCl2

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Mar 17 09:35:31 EST 1998

Hi Folks,

I want to chelate out some MgCl2 in a PCR using EDTA. It doesn't appear
to be 1:1 as expected. With 3mM Mg I can put in 3mM EDTA and it will
still work. A 1mM Mg control doesn't PCR so something is amiss. And no
it is not just as simple as making up a PCR mix with less Mg to start
with. There is a good reason for us doing it this way.

Many thanks

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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