jfujimot at oncology2.ims.u-tokyo.ac.jp
Wed Mar 18 09:51:52 EST 1998
In article <6elm22$nd at cmgm.Stanford.EDU>, jladasky at cmgm.Stanford.EDU
(John Ladasky) wrote:
> Wow. That's WAY too much sucrose. Maybe there's a decimal
> missing there? My 20X PCR-compatible loading dye recipe is 30%
> 0.30% tartrazine yellow (optional), 0.15% cresol red. So the final
> centration of sucrose in my PCR is 1.5%.
Alternatively, I usually use 5% glycerol, 0.01% cresol red (final conc.)
colony-picking PCR to check the insert. Also works well.
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