cresol ution

Jiro Fujimoto jfujimot at oncology2.ims.u-tokyo.ac.jp
Wed Mar 18 09:51:52 EST 1998


In article <6elm22$nd at cmgm.Stanford.EDU>, jladasky at cmgm.Stanford.EDU
(John Ladasky) wrote:

>         Wow.  That's WAY too much sucrose.  Maybe there's a decimal
point
> missing there?  My 20X PCR-compatible loading dye recipe is 30%
sucrose,
> 0.30% tartrazine yellow (optional), 0.15% cresol red.  So the final
con-
> centration of sucrose in my PCR is 1.5%.  

Alternatively, I usually use 5% glycerol, 0.01% cresol red (final conc.)
for 
colony-picking PCR to check the insert.  Also works well.

Regards,

Jiro Fujimoto



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