nested PCR

[Richard THIERY]

Hi all,

Any suggestions to prevent false positive results in nestedPCR reactions? I
am trying to set up such a test to detect a fish virus. Sometimes I get
some positive  reactions in my blank controls. Its seems to be due to carry
over products from the first step because I have no problem with the first
step controls.
Many thanks for your help

Richard
rthiery at ifremer.fr