dTTP -> dUTP problems in PCR

Andrew Ippolito aji97 at acsu.buffalo.edu
Wed Mar 18 19:15:20 EST 1998


just use both:)
dttp may be required for something in the reaction..
a black box.
just a thought.


Andrew Ippolito
Dept. Molecular & Cellular Biology
Roswell Park Cancer Institute
ippo at scientist.com

...why is abbreviation such a long word??


On 4 Mar 1998, Nico van Belzen wrote:

> Hi all, 
> 
> (posting this for a colleague) 
> In order to prevent contamination, I want to use dUTP/UDG (uracyl 
> glycosylase) in all diagnostic PCR systems. However, sometimes the 
> reaction is not working when I change from dTTP to dUTP. I tried to 
> increase the dUTP concentration, this did not help. Trying other 
> brands of Taq polymerase does not work. Does somebody know a solution 
> for this problem? 
> 
> Best regards, 
> 
> Nico van Belzen (belzen at hema.fgg.eur.nl) 
> 
> 
> 




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