dTTP -> dUTP problems in PCR
aji97 at acsu.buffalo.edu
Wed Mar 18 19:15:20 EST 1998
just use both:)
dttp may be required for something in the reaction..
a black box.
just a thought.
Dept. Molecular & Cellular Biology
Roswell Park Cancer Institute
ippo at scientist.com
...why is abbreviation such a long word??
On 4 Mar 1998, Nico van Belzen wrote:
> Hi all,
> (posting this for a colleague)
> In order to prevent contamination, I want to use dUTP/UDG (uracyl
> glycosylase) in all diagnostic PCR systems. However, sometimes the
> reaction is not working when I change from dTTP to dUTP. I tried to
> increase the dUTP concentration, this did not help. Trying other
> brands of Taq polymerase does not work. Does somebody know a solution
> for this problem?
> Best regards,
> Nico van Belzen (belzen at hema.fgg.eur.nl)
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