transformation efficiency

chenln at PUBLIC.BTA.NET.CN chenln at PUBLIC.BTA.NET.CN
Wed Mar 18 23:34:26 EST 1998


Thank you all for replying my question. I have another question about
electronic transformation. In my lab, there is a E.coil Pulser made by
Bio-Rad. It's voltage can be set in a range of 0-2500V. Can I use it to
transform eukariotic cells by setting up the voltage to 1500kv as Short
Protocol for Molecular Biology section 9-2(third edition)? For electronic
methode can obtain more stable recombinant than other chemical methodes.
Thank you.

chen linan

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Chen Linan               
Dept. Anatomy
Beijing Medical University
Beijing
P.R.China
Tel:86-10-63051481
Email:chenln at public.bta.net.cn 
HTTP://www.freeyellow.com/members2/chenln/

                       




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