Peptide mapping/EndoProtease Asp-N

[olivier Coqueret]

Hi,
I'am trying to do peptide mapping using the endoprotease Asp-N. Has
anyone work out the optimal conditions? Reaction buffer, pH, quantity of
enzyme and digestion time?
Many thanks (sorry for the previous message...)

Olivier Coqueret. Ph. D.                   
Molecular Oncology Group                   
McGill University
Royal Victoria Hospital
687 Pine Av. West
Montreal, Quebec
Canada  H3A 1A1
Phone: (514) 842 12 31 ex: 5832