dual luciferase assay
markus.stefan.mueller at uni-konstanz.de
Fri Mar 20 09:54:02 EST 1998
I´m going to perform some reporter gen assays with Promegas Luciferase
assay. Therefor I have got some questions to be answered. If anybody
could help I would be glad to get some helpful mails.
First, which is the best transfection method to transfekt SV80 lung
For an internal standard I intend to take advantage from the dual
luciferase system. There are vectors with encoded renilla luciferase
available under control of HSV-TK, CMV and SV40 promoters. The TK is a
relatively weak one, CMV and V40 are much more stronger. Which is the
best to use for standardazation?
Thank you very much!
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