Mutagenesis

bradley turner turner at SPRCORE.BIDMC.HARVARD.EDU
Fri Mar 20 23:19:06 EST 1998


Hi Brett,

There was recently a review article covering different methods of DNA
mutagenesis:

Approaches to DNA mutagenesis: An overview
Ling, M.M., and Robinson, B.H.
Analytical Biochemistry 254:157-178, 1997

I hope this helps,
Brad Turner

**************************************************************
  		    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
330 Brookline Avenue		bturner at bidmc.harvard.edu
Boston, MA 02215		turner at sprcore.bidmc.harvard.edu
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bbeitzel at biomail.ucsd.edu wrote:

> Hola,
>
> Just wondering if anyone had any suggestions about mutagenesis.  I have a
> cDNA that I would like to mutagenize to give me a pool of different
> mutants (point mutants and small deletions.)  Any recommendations as to
> the best way to go about doing this?
>
> thanks for any help,
> Brett Beitzel
> bbeitzel at biomail.ucsd.edu
> Infectious Disease Lab
> The Salk Institute


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