Help on Gel Filtration
slzmz at CC.USU.EDU
Mon Mar 23 01:50:29 EST 1998
I am trying to purify a small protease, which has the size about 13KDal.
What I did is that first I ran the gel filtration on Superose 12, and
then I collected the peak corresponding the activity peak. But when I run
the SDS-PAGE, I found I got a mixture of proteins. Even some pretty big
protein like 70KDal is appearing in the elution position of 13KDal. What
I did wrong?
Email: slzmz at cc.usu.edu
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