Help on Gel Filtration

Xie Yi slzmz at CC.USU.EDU
Mon Mar 23 01:50:29 EST 1998

I am trying to purify a small protease, which has the size about 13KDal. 
What I did is that first I ran the gel filtration on Superose 12, and 
then I collected the peak corresponding the activity peak. But when I run 
the SDS-PAGE, I found I got a mixture of proteins. Even some pretty big 
protein like 70KDal is appearing in the elution position of 13KDal. What 
I did wrong?

Xie Yi
Email: slzmz at

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