Analysis of 18 base oligodeoxynucleotide

Sudip K. Das das at
Sat Mar 21 23:34:07 EST 1998

I am working with a 18 base oligodeoxynucleotide (ODN) which I need to
extract in aqueous layer from chloroform - water mixture and analyze using
UV 260 method.  Unfortunately, I am getting much less amount of ODN than
what I am supposed to obtain in aqueous layer.

I would highly appreciate if someone could suggest any alternative method
and/or any explanation of the problem.

Please reply to        das at

Thanks for your help

Sudip Das
Nova Southeastern University
Ft Lauderdale, FL

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