Mutagenesis

Yoram Gerchman yoramg2 at LEONARDO.LS.HUJI.AC.IL
Mon Mar 23 10:36:51 EST 1998


You can try PCR. 
1. Do the PCR in the presence of 0.5mM MnCl2
Or
2. Use the uneven nucleotide mix method:
  a.Devide your reacion to 4 tubes, each one containing nucleotide mix 
    with one 1/100 concentration of the usual.
  b. Run for 5 cycles.
  c. Unite the content of the tubes to one and continue the run.
.........................................................................
Yoram Gerchman
Division of Microbial & Molecular Ecology
The Institute of Life Sciences
Hebrew University of Jerusalem
Givat Ram, Jerusalem 91904, Israel
Tel: 972-2-6585175




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