long pcr

steven mcfarlane smf4r at udcf.gla.ac.uk
Tue Mar 24 15:08:26 EST 1998


could anybody help me? i wish to amplify an 8kb fragment from equine
genomic DNA using Dynazyme II and i was wondering if anyone had a
protocol, ie PCR cycles and conditions etc, for such a method. i use a
perkin elmer 480 thermal cycler.

many thanks 

steven mcfarlane



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