In situ Hybridization protocols

Arati Sadalge r2d2 at bu.edu
Wed Mar 25 13:59:03 EST 1998


Working on in situs with 35S in vitro transcribed RNA probes on mouse
brain cryosections (freefloating) as well as mouse snout (slides). Have
been able to get background but NO definitive staining for months now!
Any suggestions as to protocols or places to research will be greatly
appreciated!!

A. Sadalge
r2d2 at bu.edu




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