Help on Gel Filtration

Caladan9 caladan9 at aol.com
Thu Mar 26 10:59:58 EST 1998


The contamination problem you are seeing is not unexpected, considering the
upper limit of Superose 12 is 300kD.  Perhaps Superdex 75 would be effective
depending on the size of the closest contaminate.  However, as has been pointed
out gel filtration is a poor first step.  You mention that this biomolecule is
a protease, is it a serine protease?  Often Benzamide immobilized upon a solid
support can be effective at the initial stage of purification for serine
proteases.
Gus



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