RNA extraction from pancreas

Shiao Y. Wang sywang at whale.st.usm.edu
Sat Mar 28 17:05:55 EST 1998

Corrado Cilio wrote:
> I am having trouble with RNA extraction from pancreas. I have used snap
> frozen tissue and Ultraspec kit with bad results.
> If someone has a good protocol or tips I will be very happy and
> gratefull.

Have you tried homogenizing fresh tissue directly in guanidine
thiocyanate or which ever chaotropic agent you are using? I think your
problem comes from using frozen tissue.

Shiao Y. Wang
Department of Biological Sciences
University of Southern Mississippi

More information about the Methods mailing list