RNA extraction from pancreas

Shiao Y. Wang sywang at whale.st.usm.edu
Sat Mar 28 17:05:55 EST 1998


Corrado Cilio wrote:
> 
> I am having trouble with RNA extraction from pancreas. I have used snap
> frozen tissue and Ultraspec kit with bad results.
> If someone has a good protocol or tips I will be very happy and
> gratefull.

Have you tried homogenizing fresh tissue directly in guanidine
thiocyanate or which ever chaotropic agent you are using? I think your
problem comes from using frozen tissue.

-- 
Shiao Y. Wang
Department of Biological Sciences
University of Southern Mississippi



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