How to prevent melting of short DNA duplexes

Peter Stoilov pstoilov at OBZOR.BIO21.ACAD.BG
Sun Mar 29 05:21:53 EST 1998


Hi Don,

You can try to add some PEG 8000 (up to 10%) to your reaction mixtures.
This will increase the active concentration of the oligos, respectively the
hybridisation efficiency.

Good luck.
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Peter Stoilov, PhD student
Institute of Molecular Biology
Bulgarian Academy of Sciences
G. Bonchev str. Bld21
1113 Sofia, Bulgaria
Phone: (+359 2) 713 2651	Fax: (+359 2) 723 507
E-mail: 	pstoilov at obzor.bio21.acad.bg
	pstoilov at bgearn.acad.bg
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