licor sequencing on pcr-products?

Robert G. Hamilton rhamilto at
Mon Mar 30 13:54:16 EST 1998

Coline van Moorsel wrote:
> We are seriously thinking about buying a sequencer. Which one? Licor
> does seem to have nice papers although we have heard that direct cycle
> sequencing  on PCR products seems to give problems. Anyone out there
> with similar problems?
> Thanks in advance!
> Coline
We sequenced some PCR products and had no problems. How could it make a
difference. The reactions are are a function of the thermal cycler, and
have nothing to do with the sequencer.

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