Has anyone used fluorescein-5-maleimide to label a peptide?

Kate Michie k.michie at biochem.usyd.edu.au
Tue Mar 31 01:41:31 EST 1998

I am trying to label a small peptide with fluorescein-5-maleimide (F5M)
with little success. The peptide has only one Cysteine. I have tried to
incubate the peptide and F5M in a buffer (as suggested by the chemical
company support notes) for 2 hours, however, analysis by HPLC shows little
to no yield. Has anyone else had any success? What am I doing wrong?! Any
Katharine Michie
Department of Biochemistry
University of Sydney

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