Problem with TCA precipitation

Morten Lindow Morten.Lindow.5279 at student.uu.se
Tue Mar 31 11:20:25 EST 1998


I am working with incorporation of radioactive nucleotides into the genome
of V79 hamster fibroblasts.
I want to determine the amount of incorporated activity.
I have looked into protocols for DNA precipitation with TCA, but they all
seem to be on purified DNA.
Does anyone know how to apply this method (or an alternative) to whole
cell-systems?
A quick and safe lysing maybe?

Regards,

Morten Lindow
Biomedical Radiation Sciences
Uppsala University
Sweden






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