Methods March 1998 Archives by author

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Starting: Sun Mar 1 12:22:53 EST 1998
Ending: Tue Mar 31 23:13:06 EST 1998
Messages: 870

• bidirectional fusion vector?   "", David Alexander
• cresol ution   "David Brayford ", B&B-BMB
• Plasmid Biology Meeting   International Symposium on Plasmid Biology 1998
• [Q] PAGE for total RNA   DIEGO BONATTO - 219
• cells BRL(DE3)pLysS   3Dom
• PEG PRECIPITATION OF DNA   3Dom
• [Q] RT resistance (DEPC, formamide)   최웅
• [Q] solubility of melatonin   최웅
• [Q]solubility of melatonin   최웅
• (none)   Vladimir Celer
• Biotinylation of plasmid DNA   AAAACc
• Activated Charcoal...What is it?   David Ackerley
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• Degenerate Primer Design   Tanvir Ahmad
• Degenerate Primer Design   Tanvir Ahmad
• Stable transfection of COS1   Jun-Ichi Aikawa
• Stable transfection of COS1/7   Jun-Ichi Aikawa
• hydroxylamine cleavage in GuHCl   Judith A. Airey
• Invitrogen Drosophila expression system   Philip Aisen
• maxipreps from dam- E. coli strains   Ashok Aiyar
• Generation of deoxy-uracil substituted plasmid DNA in E. coli   Ashok Aiyar
• Is it the same protein?   AlPiEs
• Tallest Sunflower Contest   Nick Albu
• Gene synthesis using PCR   Mikhail Alexeyev
• Problems with beta-Galactosidase and X-Gal   Mikhail Alexeyev
• Native polyacrylamide gel question   Bruce G. Allen
• Non-radioactive southern   Mitchell Altschuler
• Non-radioactive southern   Mitchell Altschuler
• Non-radioactive southern   Mitchell Altschuler
• non radioactive southerns   Mitchell Altschuler
• Triton X-100 precipitates   Mikkel Holmen Andersen
• EtBr in PCR; what to do? [long-ish]   John S Jacobs Anderson
• anti-HA beads??   Thomas R. Anderson
• Electroporators   Lars Andrup
• Horizontal gene transfer   Lars Andrup
• Protocol for covalently binding Protein A and Streptavidin   Asha Anil
• Bio-Rad PhosphorImager/Apple G3   Anonymous
• Protein expression in euk.cells   Shiwani Arora
• Purification of end labelled oligo probes?   ISOAI Atsushi
• sequence analysis software   Aydemir Akin, DVM
• Bio-Rad PhosphorImager/Apple G3   B.Faucon
• Bio-Rad PhosphorImager/Apple G3   B.Faucon
• Cloning problem   Stefan Backstrom
• relationship between DNA/DNA interaction and dielectricity constant   Ralf Badur
• Horizontal gene transfer   Joseph C. Bagshaw
• candidate genes   Joseph C. Bagshaw
• expression vector pSVT7   Lyrissa Baksheev
• hydroxylamine cleavage in GuHCl   James Bassuk
• His6-Tagged Protein: Binding strength, panning...   Jakob Begun
• Separation of tiny from large fragments using spin columns   Tom Belbin
• Urgent help for plasmid purification   Nico van Belzen
• heteroduplex DNA analysis   Nico van Belzen
• Gene identification troubles (bit long)   Nico van Belzen
• dTTP -> dUTP problems in PCR   Nico van Belzen
• Yeast two hybrid contamination   Matt Benson
• Antibodies against SOD in rat hepatocytes   Trond Olav Berg
• Measuring SOD activity   Trond Olav Berg
• Help: genomic DNA refused to be cut   Li Bing
• Tallest Sunflower Contest   Craig Bingman
• Purification of end labelled oligo probes?   Ransom Hill Bioscience
• JT's Restarants - Excellent Undervauled Investment Opportunity   Ransom Hill Bioscience
• (none)   Black, Michael
• Protein transfer   Shaun D. Black
• C2C12 Myotube transfection   Bertram Bleck
• Purification of end labelled oligo probes?   Fred Bloggs
• Detoxification of Etbr   Frederik Boernke
• Yeast two hybrid contamination   Frederik Boernke
• Ampicillin   Frederik Boernke
• transformation efficiency   Frederik Boernke
• transformation efficiency   Frederik Boernke
• (none)   Patrick Booms
• Bacteria deficient in all repair systems?   Eric Bouhassira
• Throw gloves after handling EtBr?   Chris Boyd
• COS Transfections - DEAE or Lipofectin?   Chris Boyd
• sequence analysis software   Deb Britt
• Triton X-100 precipitates   M. Johan Broekman
• Thermocycler/PCR-machine   Jurek Brzywczy
• Eppendorf Mastercycler gradient PCR   Jurek Brzywczy
• Phosphorylation of library proteins   Sylke Buder
• shelf life of dNTPs at -20°C   Sebastian Bunka
• Help on Gel Filtration   Markus Burger
• primers of human housekeeping gene   Maggi Burton
• RNA isolation anyone?   Maggi Burton
• primers of human housekeeping genes   Maggi Burton
• HIF primers for RT-PCR   Maggi Burton
• primers of human housekeeping gene   Maggi Burton
• primers of HIF   Maggi Burton
• primers of HIF-1alpha   Maggi Burton
• primers of 18SrRNA   Maggi Burton
• primers of 18SrRNA   Maggi Burton
• HIV-1 Tat antibody   Maggi Burton
• Labeling Proteins and Amino Acids with NBD   Dr E. Buxbaum
• Help on Gel Filtration   Caladan9
• removal of GST fusion proteins from glutathione-sepharose beads ?   Caladan9
• Labeling Proteins and Amino Acids with NBD   Phil Calvert
• Raheel Qamar   Rina Carhuaz
• candidate genes   Rina Carhuaz
• question!!!   Eliana Bustos Caro
• antibody to polyhistidine tag?   Ron Caspi
• Ecdysone inducible system: Muristerone A unavailable   Ed Castro
• COS Transfections - DEAE or Lipofectin?   Ed Castro
• Method of RT-PCR from Lysis buffer   Y Chai
• Western for Porcine Genes   Y Chai
• How to extract DNA from skin samples for PCR   Alex Chang
• c-myc expression vector   Youngil Chang
• Uneven PCR, Rapid method to walk unknown sequence, http://128.84.203.244   Xiongfong Chen
• JOB OPPORTUNITIES IN BIOTECHNOLOGY: INVESTIGATION BY NEWSGROUP.   Raoul Ciappelloni
• RNA extraction from pancreas   Corrado Cilio
• Isolation of genomic DNA from mycoplasmas   Bruce Clark
• Help! Densitometry software --Will Collage work?   Darryl L. Clark
• How to amplify 1kb PCR product   Dr. Duncan Clark
• Gene synthesis using PCR   Dr. Duncan Clark
• Gene synthesis using PCR   Dr. Duncan Clark
• LA PCR   Dr. Duncan Clark
• competitive RT-PCR   Dr. Duncan Clark
• EDTA and MgCl2   Dr. Duncan Clark
• PCR primers   Dr. Duncan Clark
• ss phage help   Dr. Duncan Clark
• ligation of BIG inserts   Dr. Duncan Clark
• antibody   Adrian Clarke
• Problem cloning PCR product into pCR-Script   Claudia
• Tallest Sunflower Contest   Mike Cohen
• CBB Stain/destain gadget   Christopher D. Coldren
• FCS (FBS) gamma-globulin free   Martin Collinson
• commercial yeast 2-hybrid screening?   Richard Converse
• Peptide mapping/EndoProtease Asp-N   olivier Coqueret
• Peptide mapping/EndoProtease Asp-N   olivier Coqueret
• Peptide Mapping/Asp-N protease   olivier Coqueret
• Detoxification of Etbr   Francois Coulier
• Que] Automated Sequencer   Marc Crepeau
• degenerate oligo PCR   Marc Crepeau
• Tet inducible mouse   Seth Crosby
• Throw gloves after handling EtBr?   Seth Crosby
• Problems with beta-Galactosidase and X-Gal   DOCTORHIM
• degenerate oligo PCR   DOCTORHIM
• Analysis of protein glycosylation?   DOCTORHIM
• Help locating ZYNAXIS   DOCTORHIM
• Pwo PCR   Nico Dantuma
• Analysis of 18 base oligodeoxynucleotide   Sudip K. Das
• genomic sourthern, help needed   David L. Haviland, Ph.D.
• baculovirus kit opinions needed   David L. Haviland, Ph.D.
• Electroporators   David L. Haviland, Ph.D.
• Poly(A)+ isolation tricks   David L. Haviland, Ph.D.
• PEG PRECIPITATION OF DNA   David L. Haviland, Ph.D.
• SiO2???   David L. Haviland, Ph.D.
• SiO2???   David L. Haviland, PhD
• Alternatives to VisiGel (Stratagene)   David W. Morris, Ph.D.
• Stratagene QuikChange Problems   Rick Davis
• Mycobacterium Libraries?   Rick Davis
• DNA isolation from cervical swabs   Victor DeFilippis
• DNA isolation from cervical swabs   Victor DeFilippis
• Gel documentation   Dean.Sequera at am.apbiotech.com
• PULSE FIELD GEL---> SOUTHERNS... HELP!?   Graham Dellaire
• Stable transfection of COS1   Graham Dellaire
• GFP fusions in E. coli   Deshpande, Raj
• Phage display against protein A immobilized target   Deshpande, Raj
• Q: what are sepabeads?   Nicola Dimitrijevic
• shelf life of dNTPs at -20°C   Benan Dincturk
• Ideal system for (plant) protein expression in yeast   Benan Dincturk
• Ideal yeast system for (plant) protein expression   Benan Dincturk
• Ideal yeast expression system for (plant) proteins   Benan Dincturk
• Horizontal gene transfer   Benan Dincturk
• Cloning cDNA directly from PCR products   Benan Dincturk
• Codon usage:What does E.coli do when it can not sufficient tRNAs necessary for the heterologous protein?   Benan Dincturk
• In vitro translation systems   Benan Dincturk
• GFP cell clone   Qunfeng Dong
• two hybrids for tubulin interactions   Thomas Duchaine
• Help again for plasmid purification!   Richard J. Dudley
• How to amplify 1kb PCR product   Richard J. Dudley
• Urgent help for plasmid purification   Richard J. Dudley
• Stratagene QuikChange Problems   Richard J. Dudley
• sequence analysis software   Richard J. Dudley
• Looking for a good in vitro translation kit.   Richard J. Dudley
• [Q] RT resistance (DEPC, formamide)   ELADER at AMBION.COM
• PCR probe labeling   ELADER at AMBION.COM
• competitive RT-PCR   ELADER at AMBION.COM
• competitive RT-PCR   ELADER at AMBION.COM
• Subcloning problems   ELADER at AMBION.COM
• Subcloning problems   ELADER at AMBION.COM
• long-PCR   Christian Ebeling
• antibody to polyhistidine tag?   David M. Eisenmann
• longpcr   David M. Eisenmann
• thermo-cycler   David M. Eisenmann
• (Kein "Betreff")   Entwicklung
• peptide design question   Eric S. Bean, PhD
• hydroponic culture of arabidopsis   Dietrich Ernst
• Q: Transfection control plasmid   Eugene
• GFP or luciferase as marker for gene transfer???   Eugene
• Listmode data conversion: from Elite to Beckon format.   Eugene
• Restriction enzymes study   Joan Evans
• Scientific & Medical Journals on the Web   Joan Evans
• Upcoming Study on RNA Isolation   Joan Evans
• Electroporators   Judith Blasser F-H
• Pwo PCR   Frank O. Fackelmayer
• bioinformatic   Eric Fairfield
• Cucurbita maxima DNA library?   Peter C Farley
• purification of ssDNA   Susanne Felder
• End labelling a primer   John Fellers
• Cold EMSA   Luc Ferrari
• Yeast 2 hyb screening   S. Findley
• Afl II ligations... any suggestions?   Karl Fischer
• Help: genomic DNA refused to be cut   Karl Fischer
• sequencing PCR products   Karl Fischer
• Muristerone A   Karl Fischer
• Single-Stranded Rescue Procedure   Karl Fischer
• How to remove Sigmacote from glass plate?   Karl Fischer
• Cloning problem   Karl Fischer
• Subcloning problems   Karl Fischer
• ss phage help   Karl Fischer
• Help on Gel Filtration   Karl Fischer
• B12 mutant strain   Karl Fischer
• HIV-1 Tat antibody   Brian Foley
• shelf life of dNTPs at -20°C   Bryan L. Ford
• thermo-cycler   Bryan L. Ford
• cresol ution   Jiro Fujimoto
• about PCR   GENYAN
• promoter analysis   ANNE GEORGE
• extra G in 5' RACE   Warren Gallin
• Q: remove enzyme with antiserum?   Warren Gallin
• choice of probes   Ganja
• Proposal for a new bionet newsgroup   GcR
• GFP or luciferase as marker for gene transfer???   GcR
• S1/Mung Bean   Dr. Peter Gegenheimer
• Rates of DNA Synthesis with Thermostable Enzymes   Dr. Peter Gegenheimer
• Mutagenesis   Yoram Gerchman
• Primer protection in random mutagenesis   Yoram Gerchman
• Identifying candidate genes   Jan Geurts
• problems with reverse primer   Mohammad H Ghahremani
• Yeast 2 hyb screening   Sourav Ghosh
• Air gel dry   Gizbab
• sequencing PCR products   Gizbab
• Bisulfite and DNA methylation   Gizbab
• sequence analysis software   Gizbab
• fungal dna extraction   Michelle Gleeson
• Microsatellite amplification - parasite bands   Pawe³ Golik
• Spleen cell proliferation assays   J.S Good
• Biotinylation of plasmid DNA   J.S Good
• Poly-HIS binding peptides/proteins   Mark Gosink
• hydroxylamine cleavage in GuHCl   Roney Graf
• Ubiquitous primers   Richard P. Grant
• Throw gloves after handling EtBr?   Richard P. Grant
• shelf life of dNTPs at -20°C   Richard P. Grant
• Urgent help for plasmid purification   Richard P. Grant
• shelf life of dNTPs at -20°C   Richard P. Grant
• Ubiquitous primers   Richard P. Grant
• Ubiquitous primers   Richard P. Grant
• ? alternative for Pharmacia silanes ?   Richard P. Grant
• maxipreps from dam- E. coli strains   Richard P. Grant
• ethidiumbromide fate   Samuel Gray
• Ribosomal RT-PCR control primers   Samuel Gray
• Sequencing gels -bubbles   Mike Gruidl
• gene identification troubles   R.D. Haigh
• Autoclaved MOPS: is it supposed to be yellow?   Deborah Hailstones
• IMS-PCR   Deborah Hailstones
• Problems with electrotransformation of E. coli TOP10   Michael Halford
• Hot Start Wax Beads   Alastair Hamilton
• licor sequencing on pcr-products?   Robert G. Hamilton
• primers of human housekeeping gene   Han
• genomic digestion   Han
• degenerate oligo PCR   Han
• test   Vinh Hang
• BrdU antibody POD conjugated ?   Jens Rainer Hansen
• Q: Protein expression in yeast   Dimitri Harmegnies
• looking for an antibody library   Dimitri Harmegnies
• Muristerone A   Michele Harvey
• Invitrogen Drosophila expression system   Michele Harvey
• upstream seq by PCR   Randy Haun
• Vanillin - Lipids????????????   Ian Hayman
• Hot Start Wax Beads   Anthony Heape
• FCS (FBS) gamma-globulin free   John Hegarty
• Tallest Sunflower Contest   Pieter Heijms
• Autoclaved MOPS: is it supposed to be yellow?   J. Hendriks
• vector pSVT7   Susanna Herd
• Auto Sequencing in Ireland   Jim Hill
• Good enzymes for cutting vertebrate cDNA   Jim Hill
• Poly(A)+ isolation tricks   Jim Hill
• PCNA   Hung-yiu Ho
• sequencing PCR products   Miss. V.M. Hodges
• primers for leukaemic translocations   Miss. V.M. Hodges
• licor sequencing on pcr-products?   Kerstin Hoef-Emden
• Throw gloves after handling EtBr?   Susan Jane Hogarth
• Throw gloves after handling EtBr?   Annette C. Hollmann
• Genexpress-Genethon IMAGE clones unobtainable?   Mike Holloway
• Clontech Keratinocyte library   N A Hotchin
• Air gel dry   Ken Howe
• erythromycin   Ke-Xue Huang
• B12 mutant strain   Ke-Xue Huang
• Compagnies for mmp   Eric Huet
• Spots on Northerns?   Barclay Morrison III
• DNA Tissue Yield   Barclay Morrison III
• Help: genomic DNA refused to be cut   Mathieu Ingouff
• thermo-cycler   Mathieu Ingouff
• size of ribosomal RNAs ?   Wendy Ingram
• size of ribosomal RNAs ?   Wendy Ingram
• Poly(A)+ isolation tricks   Wendy Ingram
• DNA fragments extraction with NaI   Wendy Ingram
• dTTP -> dUTP problems in PCR   Andrew Ippolito
• advice about non radioactive kit for DNA labelling   Irina
• Bio-Rad PhosphorImager/Apple G3   Michael Irwin
• Mouse strain expressing the tetracycline repressor   Ramaswamy K. Iyer
• metabolic labeling of proteins in e.coli...   Sai Iyer
• metabolic labeling of proteins in e.coli update...   Sai Iyer
• Detection of tritiated ligand   Fraser Dr N J
• Biotinylation of plasmid DNA   Fraser Dr N J
• MacVector upgrade and GeneWorks   JDeikman
• protein Sequencing   ITABAJARA DA SILVA VAZ JUNIOR
• Cloning cDNA directly from PCR products   Christophe Jacob
• Sequencing gels -bubbles   Martin Jakt
• peptide design question   Christopher Jarvis
• msp I digestion   Dr GJS Jenkins
• promoter compatibilty   Ju-Hong Jeon
• ligation of BIG inserts   Boris Jerchow
• postdoc. position   William Jia
• postdoc. position   William Jia
• question   Jose Alfredo Iglesias Jimenez
• Problems with beta-Galactosidase and X-Gal   J. Jockers
• Rates of DNA Synthesis with Thermostable Enzymes   Doug Johnson
• karyotype   Randall Johnson
• Ampicillin   Jillian Johnston
• Native polyacrylamide gel question   Zophonias O. Jonsson
• transformation efficiency   Zophonias O. Jonsson
• (none)   Salih KAFKAS
• method for genetic inheritance between parent and F1   Salih KAFKAS
• Contaminated -Taq   D. KIM
• Thermocycler/PCR-machine   D. KIM
• Mutagenesis   D. KIM
• Primary chicken antibodies?   Chris Kafer
• Proposal for a new bionet newsgroup   Jim Kami
• Prokaryotic expression vector pKK223-2   Yahya Kargalioglu
• Gene synthesis using PCR   Geoffrey Kidd
• PCR with low specific cDNA concentration   Geoffrey Kidd
• [Q] about limiting dilution   Kim, Cheon
• [Q] about limiting dilution   Kim, Cheon
• [Q] methylne blue .vs. acetocarmine   Kim, Cheon
• [Q] primer design   Kim, Cheon
• Que] Automated Sequencer   Hyunmin Kim
• Listmode data conversion: from Elite to Beckon format.   Jorg Kirberg
• Detoxification of Etbr   Jongrak Kittiworakarn
• Asking about AEC   Jongrak Kittiworakarn
• Asking about transferring condition   Jongrak Kittiworakarn
• Help. Factor Xa digestion   Jongrak Kittiworakarn
• Re; Ampicillin   Jongrak Kittiworakarn
• Subcloning problems   Jongrak Kittiworakarn
• Stability of S-hexylglutathione agarose?   Jongrak Kittiworakarn
• Buffer with pH10   Jongrak Kittiworakarn
• Help...GST folding   Jongrak Kittiworakarn
• CBB Stain/destain gadget   Jongrak Kittiworakarn
• Inverted epitope from peptide libraries   Svend Kjfr
• Need to start baculovirus work. Please give your recommendations on kits and protocols   Dima Klenchin
• PEA/PPEA   William Klimke
• Proposal for a new bionet newsgroup   Lars Komorowski
• AFLP's   John Korte
• extra G in 5' RACE   Gae Kovalick
• Detoxification of Etbr   Cornelius Krasel
• Help on Gel Filtration   Cornelius Krasel
• B12 mutant strain   Rich L
• Cloning cDNA directly from PCR products   Richard L
• PCR primers   Richard L
• Commercial Mass Spec   Wood Lab
• dTTP -> dUTP problems in PCR   John Ladasky
• Clone with Alu repeats - how to sequence?   John Ladasky
• LA PCR   John Ladasky
• cresol ution   John Ladasky
• DNA Extraction from CSF   Ladriere
• Q:PCR labeled with 32P?   Lai
• Phage display against protein A immobilized target   Ralf Landgraf
• DNA fragments extraction with NaI   Denis M. Larkin
• Chemiluminescent nucleic acid detection kit   Denis M. Larkin
• ABI 310 Genetic analyzer running buffer   Flemming Larsen
• ss phage help   Rob Latch
• Allele-specific PCR - Am I working too hard?   Andy Law
• pRK5 Expression Vector   Sam Lee
• ethidiumbromide fate   Sam Lee
• Need to start baculovirus work. Please give your recommendations on kits and protocols   Sam Lee
• His-tagged proteins   Andrew Leech
• Air gel dry   Kai Lehmann
• Need anti-human CD4 ab that works on paraffin sections   David N. Levy
• Autoclaved MOPS   Richard Lightfoot
• Poly(A)+ isolation tricks   Richard Lightfoot
• Help! Antibody for yeast alpha factor signal peptide   Feng Lin
• ligation of BIG inserts   Tzu-Ping Lin
• Growing BAC cultures successfuly   Tzu-Ping Lin
• Q: 3'UTR sequence programs   Jonas Lindeberg
• Problem with TCA precipitation   Morten Lindow
• degenerate oligo PCR   J. Casey Lippmeier
• degenerate oligo PCR   J. Casey Lippmeier
• Electroporation of sea urchin eggs   Hector Lucero
• A Novel Software of Organic Mass Spectra   Yu-Ran Luo
• transformation efficiency   R. John Lye
• oligonucleotide ligation   RICHARD MARTIN
• antibody to polyhistidine tag?   RICHARD MARTIN
• Multiplex PCR from hairs   David MacHugh
• PLEASE HELP...RNA/protein binding   Bill Mackinaw
• nested PCR   Ulrich Maier
• DNA Extraction from CSF   Ulrich Maier
• size of ribosomal RNAs ?   Frank Maiwald
• size of ribosomal RNAs ?   Frank Maiwald
• size of ribosomal RNAs ?   Frank Maiwald
• size of ribosomal RNAs ?   Frank Maiwald
• ethephone concentration ?   Frank Maiwald
• Primer Adapters and PCR   Greg and/or Mandy
• ADN-protein electroelution ?   Alexandra Manevski
• S1/Mung Bean   Ned Mantei
• Growing BAC cultures successfuly   Laura F Marek
• Subcloning problems   Cynthia Marie-Claire
• Native polyacrylamide gel question   Mike Marr
• BIO-TECH CHEMICALS - banr.gif (0/1)   Todd Martinsky
• microarray biochip picture   Todd Martinsky
• (none)   Jeremy Martinson
• 15N and 13C labelled proteins from baculo systems?   Ari Marttila
• THP-1 transfection   Simon Mauch
• Help on Gel Filtration   Ian McFarlane
• Human probes on hamster Northern   Ian McFarlane
• hydroxylamine cleavage in GuHCl   Scott McMahan
• hydroxylamine cleavage in GuHCl   Scott McMahan
• hydroxylamine cleavage in GuHCl   Scott McMahan
• Biologic from Biorad   Hassane Mchaourab
• HSC70 gene   Menoret
• request reagent   Menoret
• Asking about AEC   Menoret
• pUC10   Menoret
• relationship between DNA/DNA interaction and dielectricity constant   Dr. David J. Meyer
• (no subject)   Sam Michaelson
• theory of transgenic/knockout mouse production?   Sam Michaelson
• Has anyone used fluorescein-5-maleimide to label a peptide?   Kate Michie
• Sequencing gels -bubbles   Robert M. Mihalek
• Primers for Helicobacter   Andrew Mikosza
• DNA Probe Detection by ELISA   Jeff Miner
• licor sequencing on pcr-products?   Coline van Moorsel
• Who can help me about pJLA503?   Daniel Morris
• StEP PCR   Dr Daniel Morris
• protocol for JM109 competent cell   Tha Mouse
• dual luciferase assay   Markus Mueller
• UK supplier of microwave ovens.   Vincent Mulholland
• competitive RT-PCR   Fern E. Murdoch
• here comes the hot StEPa   Jeremy M. Murray
• Mutagenesis   Jeremy M. Murray
• Mutagenesis   Jeremy M. Murray
• StEP PCR   Jeremy M. Murray
• antibody to polyhistidine tag?   Per Mygind
• methylcytosine mapping   NH at LGC.CO.UK
• methylcytosine mapping -Forwarded   NH at LGC.CO.UK
• protocol for JM109 competent cell   Name
• Afl II ligations... any suggestions?   Name
• Peptide mapping/EndoProtease Asp-N   Alain Nepveu
• removal of GST fusion proteins from glutathione-sepharose beads ?   Mark Nicholls
• HPLC Hitachi help me   Claus S. Nielsen
• E.coli intracellular metabolites   Santosh Noronha
• Thermocycler/PCR-machine   John O'Brien
• erythromycin   Michael O'Donohue
• Bacillus expression   Michael O'Donohue
• False positives?   Michael O'Donohue
• Bacillus expression vector   Michael O'Donohue
• PTK domains ID?   ERNESTO OVIDEO ORTA
• PTK domains ID?   ERNESTO OVIDEO ORTA
• Test   ERNESTO OVIDEO ORTA
• (none)   ERNESTO OVIDEO ORTA
• nested PCR   Elizabeth Ojeda
• Q: Extremely unspecific oligo   Ralf Oltmanns
• Q: Extremely unspecific oligo   Ralf Oltmanns
• [Q] PAGE for total RNA   Ralf Oltmanns
• [Q] RT resistance (DEPC, formamide)   Ralf Oltmanns
• Que] Automated Sequencer   Ralf Oltmanns
• question!!!   Ralf Oltmanns
• question!!!   Ralf Oltmanns
• Need to start baculovirus work. Please give your recommendations on kits and protocols   Onan
• PTKs domains ID?   Ernesto Oviedo Orta
• Protein transfer   Stephane Ory
• New EtBr Management Products   Mathew Osborn
• Need anti-human CD4 ab that works on paraffin sections   Bjorn P.
• IgM purification   Bjorn P.
• New and interesting site for antibodies.   Bjorn P.
• DNA quantitation : what a pain   June Eva Paciga
• primers of 18SrRNA   June Eva Paciga
• The Antibody Resource Page   The Antibody Resource Page
• Arginine Assay   Jeg Palras
• Arginine Assay   Jeg Palras
• His-tagged proteins   Dr Dave Parcej
• Hybond N+   Mr R.J. Parkes
• PCR problem   Mr R.J. Parkes
• S17 mini preb   Lestrate Pascal
• phagosome purification   Lestrate Pascal
• [Q] RT resistance (DEPC, formamide)   Lestrate Pascal
• How to determine the sex of the rat embryo   Pasqualini
• Lung proteins   R Patel
• RT-PCR Protocols.   Shiv Patel
• Yeast and GFP   Paula
• BMB's quick ligation kit   John J Peloquin
• PCR primers   Penguin
• Northern blot with non-radioactive probes   Joana Perdigao
• (none)   Joana Perdigao
• silver staining problem   Peter
• sequence analysis software   Peter
• EGTA   Peter
• Buffer with pH10   Peter
• EGTA   Peter
• Matrigel RNA extraction   Peter
• Problem with TCA precipitation   Peter
• PC12 cells   Emmanuel Petroulakis
• [Q] PAGE for total RNA   A. Piccin
• HCOOH cross linked proteins...   Belhumeur Pierre
• Ligation and electroporation   Markus Piotrowski
• EDTA and MgCl2   Markus Piotrowski
• Codon preference in plants   Markus Piotrowski
• GFP and protein interaction?   Markus Piotrowski
• Help: antibodies against MAP2, Laminin, CSPG in newborn rats   J.P. Poindessault
• Gas Cell Disruption vs French Press   Neil Pomroy
• Has anyone used fluorescein-5-maleimide to label a peptide?   Neil Pomroy
• (none)   Pavel Pospisil
• ABIC '98 - International AgBiotech Conference (Fwd.)   C. S. Prakash
• erythromycin   Steve Qi
• eGFP and autofluorescence   Paul Quax
• eGFP and autofluorescence   Paul Quax
• PCNA   Jean-Paul RAFFIN
• Proposal for a new bionet newsgroup   RManns7
• Successful phagemid excision from Zap-express ???   Ravi Ramachandran
• End labelling a primer   Ravi Ramachandran
• ? alternative for Pharmacia silanes ?   Alison Reavley
• Codon preference in plants   Mary P. Remington
• Radiolabeling of Proteins using Protein Kinase A   Blair R. Renshaw
• Human 1st strand cDNA Panels?   Blair R. Renshaw
• Charcoal stripped FCS   Rentia
• PCRing on Bacterial Genomic DNA [summary]   Stuart Rison
• Help locating ZYNAXIS   Terry Riss
• His-tagged proteins   Rob
• Antibody production   Rob
• Extracellular matrix analisys   Roberto
• Mutagenesis   Kim Roberts
• Thermal cyclers   Sarah Roberts-Thomson
• Air gel dry   Sarah Roberts-Thomson
• Commercially avail phosphohistidine or Phosphoaspartate Ab?   Sean Rollins
• Commercial PhosphoHis or PhosphosAsp Ab?   Sean Rollins
• Commercial PhosphoHis or PhosphoAsp Ab?   Sean Rollins
• ECACC   Michael Rosemann
• Bio-Rad PhosphorImager/Apple G3   Elizabeth Rosenberg
• Need anti-human CD4 ab that works on paraffin sections   Steffen Roth
• antibody to polyhistidine tag?   Steffen Roth
• Separation of tiny from large fragments using spin columns   Hiranya Roychowdhury
• Autoclaved MOPS: is it supposed to be yellow?   Hiranya Roychowdhury
• How to amplify 1kb PCR product   Hiranya Roychowdhury
• Native polyacrylamide gel question   Hiranya Roychowdhury
• How to remove Sigmacote from glass plate?   Hiranya Roychowdhury
• (none)   Hiranya Roychowdhury
• EDTA and MgCl2   Hiranya Roychowdhury
• DNA from paraffin-embedded tissue-a good control gene   Zbigniew Rudzki
• ? alternative for Pharmacia silanes ?   Zbigniew Rudzki
• transformation efficiency   SJ Rulli
• Cloning cDNA directly from PCR products   George Rutherford
• ? alternative for Pharmacia silanes ?   George Rutherford
• FW: small help   P.E. Sado S9652795
• How to determine the sex of the rat embryo   SDWOLPE
• COS Transfections - DEAE or Lipofectin?   SDWOLPE
• Bacillus expression   SHENGYANG
• Bacillus expression   SHENGYANG
• Bacillus expression vector   SHENGYANG
• Bacillus expression vector   SHENGYANG
• EGTA   SHENGYANG
• SEQUENCING GELL   MEHBOOB SHIVJI
• labelling G-3-P   CHRIS SMALL
• In situ Hybridization protocols   Arati Sadalge
• In situ techniques   Arati Sadalge
• Phage display against protein A immobilized target   Saltman at neb.com
• Serum for cell culture   Tony Sanchez
• Muristerone A   Heinz J. Schaefers
• How long in vitro transcripts?   Heinz J. Schaefers
• about pcr   Wolfgang Schechinger
• Q: Transfection control plasmid   Wolfgang Schechinger
• PCRing on Bacterial Genomic DNA [summary]   Wolfgang Schechinger
• Detection of farnesylation and other lipd modifications of prote   Wolfgang Schechinger
• Mitochonbdrial import sequence   Wolfgang Schechinger
• Ultroser and Growth Factors?   Wolfgang Schechinger
• Proposal for a new bionet newsgroup   Wolfgang Schechinger
• ? alternative for Pharmacia silanes ?   Wolfgang Schechinger
• Arginine Assay   Wolfgang Schechinger
• Proposal for a new bionet newsgroup   Wolfgang Schechinger
• Is it the same protein?   Wolfgang Schechinger
• Codon preference in plants   Wolfgang Schechinger
• Tallest Sunflower Contest   Wolfgang Schechinger
• peptide design question   Wolfgang Schechinger
• thermo-cycler   Wolfgang Schechinger
• HRP substrate   Wolfgang Schechinger
• vaccinia DNA purification kit   Wolfgang Schechinger
• Problem with TCA precipitation   Wolfgang Schechinger
• Charcoal stripped FCS   A. Schmiechen
• ethidiumbromide fate   Peter Schuchert
• Help locating Molecular Research Reagents Inc.   Andrew Scott
• EtBr in PCR; what to do? [long-ish]   John Richard Seavitt
• Where to find silica particles for dna extraction of skeletal remains?   Biochemical Sciences Section
• "Nuclei Run-On Assay"-need info   Serg
• Urgent help for plasmid purification   Todd Serulneck
• Help again for plasmid purification!   Todd Serulneck
• Urgent help for plasmid purification   Todd Serulneck
• LA PCR   Paola Sgado
• RNA isolation anyone?   Jonathan Shillingford
• RT-PCR technique   Jonathan Shillingford
• RNA isolation anyone?   Jonathan Shillingford
• primers of human housekeeping gene   Jonathan Shillingford
• 'Amplification Assistant'   Jonathan Shillingford
• Q: 3'UTR sequence programs   Jonathan Shillingford
• PTKs domains ID?   Jonathan Shillingford
• PCR on the Web   Jonathan Shillingford
• On-line manual   Jonathan Shillingford
• Pwo PCR   Jonathan Shillingford
• Ask for help on RT-PCR   Jonathan Shillingford
• Gene synthesis using PCR   Jonathan Shillingford
• baculovirus kit opinions needed   Michael Skidmore
• heteroduplex DNA analysis   Heyko Skladny
• DNase the RNA!   Coby Slagter
• Throw gloves after handling EtBr?   Koen De Smet
• ABI 310 Genetic analyzer running buffer   Koen De Smet
• Problem cloning PCR product into pCR-Script   Koen De Smet
• Q: remove enzyme with antiserum?   Koen De Smet
• EGTA   Wendy-Anne Smith
• Successful phagemid excision from Zap-express ???   Smoothies
• gel documentation systems   Smoothies
• size of ribosomal RNAs ?   David F. Spencer
• [Q] RT resistance (DEPC, formamide)   David F. Spencer
• SiO2???   David F. Spencer
• Selection for large inserts in plasmids?   Dom Spinella
• IgM purification   Dom Spinella
• Phage display against protein A immobilized target   Dom Spinella
• transformation efficiency   Dom Spinella
• How to prevent melting of short DNA duplexes   Dom Spinella
• COS Transfections - DEAE or Lipofectin?   Dom Spinella
• COS Transfections - DEAE or Lipofectin?   Dom Spinella
• cloning - help!!!!! hello people! we have a cloning problem in our lab and hope someone else out there may have come across something similar/some ideas (if this ng is inappropriate - sorry!). OK, we are trying to clone a 2kb fragment into a   Dom Spinella
• Expression Problems   Henri M.H. Spronk
• yeast strain wanted   John Stebbins
• Pwo PCR   Bob Steinberg
• sequencing PCR products   Bob Steinberg
• Thermal cyclers   Bob Steinberg
• Tallest Sunflower Contest   Steve
• How to prevent melting of short DNA duplexes   Steve
• Antibody Purification: better method than western technique?   Grant Stewart
• Muristerone A   Grant Stewart
• How to prevent melting of short DNA duplexes   Peter Stoilov
• I've had it with jtsr-stock@jtsr-stock.com!   Erik M Stokhof
• Best UV-Vis photometer   Andreas Stolpe
• Ubiquitous primers   Allan Strand
• gel documentation systems   Allan Strand
• Matrigel RNA extraction   Succinate
• Ubiquitous primers   Steven Sullivan
• Transgenic Nicotiana benthamiana plants with CAT gene   Fengcheng Sun
• sequencing gel apparatus   William Sun
• Yeast 2 hyb screening   Vladimir Svetlov
• His6-Tagged Protein: Binding strength, panning...   Vladimir Svetlov
• Brent/Clontech yeast 2-hybrid screen: recombination/rearrangements.   Vladimir Svetlov
• Gene identification troubles (bit long)   Vladimir Svetlov
• Codon usage:What does E.coli do when it can not sufficient tRNAs necessary for the heterologous protein?   Vladimir Svetlov
• antibody to polyhistidine tag?   Vladimir Svetlov
• Mutagenesis   Vladimir Svetlov
• Subcloning problems   Vladimir Svetlov
• Tallest Sunflower Contest   Vladimir Svetlov
• pRK5 Expression Vector   Geoffrey Swanson
• dTTP -> dUTP problems in PCR   Richard THIERY
• nested PCR   Richard THIERY
• sequence analysis software   Dennis Templeton
• Ampicillin   Dennis Templeton
• DNA binding by GAL4-fusion proteins   Kannan Thiru
• DNA binding by GAL4-fusion proteins   Kannan Thiru
• Anyone doing proteomics research?   Carol Thomas
• E. Coli strains for high copy number   Jay Thompson
• Spots on Northerns?   John R. Thompson
• Yeast and GFP   John R. Thompson
• GFP or luciferase as marker for gene transfer???   John R. Thompson
• [Q] RT resistance (DEPC, formamide)   John R. Thompson
• Selection for large inserts in plasmids?   John R. Thompson
• ignore: test post   John R. Thompson
• oligonucleotide ligation   John R. Thompson
• Urgent help for plasmid purification   Thomas Tomzynski
• QUANTIFICATION OF PARAQUAT   Jorge Hernandez Torres
• How long in vitro transcripts?   Marek Treiman
• DNA Ladering for Apoptosis   S.H. Tschang
• DNA binding by GAL4-fusion proteins   Darren Tyson
• Microplate reader recommendations?   M. D. Unson
• Stratagene QuikChange Problems   RHS Linux User
• PLASMID BIOLOGY 1998 MEETING   Edgar Valencia
• LDPE dialysis membrane   Sylvain Versailles
• RT-PCR Protocols.   Vidar
• HRP substrate   Vilim
• Transposon sequencing strains   Vilim
• Ligation and electroporation   Arnoud van Vliet
• help w/ fluorescin GENE IMAGES kit   Arnoud van Vliet
• help w/ fluorescin GENE IMAGES kit (part 2)   Arnoud van Vliet
• ClustalX does not work in Microsoft Windows 95.   Arnoud van Vliet
• Ecoli strains chlA (moaD gene), chlN (moeB) and chlM (moaE)   P. Vreken
• long pcr   WSchick
• New CC method with large batches, all samples tagged: developer wanted   S. Wadman
• New CC method with large batches, all samples tagged: developer wanted   S. Wadman
• help w/ fluorescin GENE IMAGES kit   Paul Wakenight
• Reverse Transcriptase--unit definition, price   Don Wang
• Gel documentation   Shiao Y. Wang
• gel documentation systems   Shiao Y. Wang
• RNA extraction from pancreas   Shiao Y. Wang
• upstream seq by PCR   Andrew Warkman
• DNA quantitation : what a pain   Krzysztof Wasowicz
• ? alternative for Pharmacia silanes ?   Krzysztof Wasowicz
• Spleen cell proliferation assays   K. Weber
• Novagen cDNA Library Kit   Jun-Zhi Wei
• RNA marker doesn't show up on formaldehyde gel   Jun-Zhi Wei
• two hybrids for tubulin interactions   Ralf Weigel
• Purification of end labelled oligo probes?   January Weiner
• SEQUENCING GELL   January Weiner
• RNA isolation anyone?   January Weiner
• RNA isolation anyone? - ROTH website   January Weiner
• ? alternative for Pharmacia silanes ?   January Weiner
• End labelling a primer   January Weiner
• Our_view_on_NATO___________[ignore]   January Weiner
• RT-PCR technique, Re:Long distance (RT) PCR   Mr. T. Weissensteiner
• Give me a hand   Zhou Wenchao
• Pharmacia PCR Pimers - Caveat Emptor   Linda Western
• fluorometry standard   C.S. Wilding
• DNA Extraction from CSF   Jenny Williams
• DNA extraction from CSF   Jenny Williams
• Single-Stranded Rescue Procedure   Wilson
• How to remove Sigmacote from glass plate?   Wilson
• Analysis of protein glycosylation?   Harry Witchel
• Primers for mouse cyclophilin or gapdh?   Harry Witchel
• yeast three hybrid   Jonny Wood
• request for gibbon genomic DNA library   Ms EA Worthey
• request for gibbon genomic DNA library   Ms EA Worthey
• THP-1 transfection   XXX
• Thanks   Xiao-Hua Xue
• thanks   Xiao-Hua Xue
• looking for R7402, can anyone help?   Hong Yan
• In situ hybridization buffer   Timur Yarovinsky
• Help on Gel Filtration   Xie Yi
• H pylori toxin VacA source?   Ian A. York
• Heat-shock protein protocols   Ian A. York
• Stable transfection of COS1/7   Ian A. York
• I've had it with jtsr-stock@jtsr-stock.com!   Ian A. York
• COS Transfections - DEAE or Lipofectin?   Ian A. York
• myc & max expression vector   Chang Youngil
• ClustalX does not work in Microsoft Windows 95.   Zhongtang Yu
• Looking for a good in vitro translation kit.   Qiaoping Yuan
• Electroporation of sea urchin eggs   Robert W. Zeller
• upstream seq by PCR   Jun Zhang
• competitive RT-PCR   Jun Zhang
• PCR problem   Jun Zhang
• bioinformatic   Jun Zhang
• primer design   Jun Zhang
• vaccinia DNA purification kit   Manchao Zhang
• Urgent help for plasmid purification   Marieke R. Koedood Zhao
• PCR probe labeling   Tiejun Zhao
• Non-radioactive southern   X. Y. Zheng
• Hybond N+   X. Y. Zheng
• msp I digestion   X. Y. Zheng
• msp I digestion   X. Y. Zheng
• protocol for JM109 competent cell   Dr. Zhao Zhuoshen
• Biotech game!   a-taq
• Gene screen   ad9341 at wayne.edu
• competent bacteria   alvaro at cephb.fr
• sarkosyl   anonymous
• vector to clone cDNA downstream of an HA tag?   aparn at ingress.com
• thermo-cycler   bartoszewski
• Fundaments on SSR's   bb1rofra at uco.es
• Mutagenesis   bbeitzel at biomail.ucsd.edu
• Gene synthesis using PCR   brett
• How long in vitro transcripts?   brett
• Native polyacrylamide gel question   ccc6 at Lehigh.EDU
• Affymetrix   cgriffin at wco.com
• (none)   chenln at PUBLIC.BTA.NET.CN
• transformation efficiency   chenln at PUBLIC.BTA.NET.CN
• IP   alina cures
• Ideal system for (plant) protein expression in yeast   bipin k. dalmia
• competitive RT-PCR   helene dauchel
• COS Transfections - DEAE or Lipofectin?   delios97 at hotmail.com
• COS Transfections - DEAE or Lipofectin?   delios97 at hotmail.com
• Post-Doc Position in bacterial/yeast metabolic engineering   dienb at ncaur.usda.gov
• RT-PCR technique   alex dobrovic
• Gel documentation set ups-recommendations please.   alex dobrovic
• Help! Densitometry software --Will Collage work?   drdad at ktc.com
• QiAmp Blood Kit   efrost6 at yahoo.com
• Gene identification troubles (bit long)   tammy english
• anti-HA beads??   erikhom at cgl.ucsf.edu
• SEQUENCING GELL   Crepineau florent
• Cloning problem   g4037881 at STUDENT1.MAHIDOL.AC.TH
• need pJM17 map   gastro
• Antibody for ATM gene product   gavin.turbett at rph.health.wa.gov.au
• Cloning problem   gmclab221
• PULSE FIELD GEL---> SOUTHERNS... HELP!?   mrs mary gosal
• How to amplify 1kb PCR product   gzzhjr
• Conference Announcement   gzzhjr
• pCAL or CaM-binding Peptide fusion proteins   hT
• Where to find silica particles   James habgood
• DNA fragments extraction with NaI   James habgood
• RNA prep from gram+ bacteria???   harald at mol.univie.ac.at
• cloning - help!!!!!   adrian hardy
• DNA contamination   xudong huang
• BIO-TECH CHEMICALS - banr.gif (0/1)   hung at well.org
• Neisseria gonrrohoeae PCR   i00001 at STUHI.PTF.HWB.NL
• maxipreps from dam- E. coli strains   jheus at pharming.nl
• JT's Restarants - Excellent Undervauled Investment Opportunity   jtsr-stock at JTSR-STOCK.COM
• quantification of cDNA after RT-PCR   Shabaana Abdul khadar
• RNA extraction from paraffin embedded tissues   Shabaana Abdul khadar
• commercial luciferase vectors?   kosiewicz at packardinst.com
• Your Web Site Re-Launch   launch at LAUNCHMASTER.COM
• Protocol for covalently binding Protein A and Streptavidin   lbergelson at worldnet.att.net
• sensitivity of methylene blue staining ?   s lecair
• hydroxylamine cleavage in GuHCl   loick.ledantec at hol.fr
• silver staining problem   jose manaligod
• gel documentation systems   jose manaligod
• Need anti-human CD4 ab that works on paraffin sections   mark
• FREE GUIDES   masse at til.es
• long pcr   steven mcfarlane
• longpcr   steven mcfarlane
• long pcr   steven mcfarlane
• PCR with low specific cDNA concentration   mnigon at caoba.entelnet.bo
• $.03 PHONE CALLS?   nancyloo at juno.com
• Buffer with pH10   thu nhat thi nguyen
• cell fractionation method?   noone
• Antibody production   noone
• Q: remove enzyme with antiserum?   noone
• cRACE mapping of 5'ends bacterial mRNAs   nooned at tcd.ie
• acetamide   nrimc
• Has anybody knows what is the extinction coefficent for tetracycline-HCl (Water solution), afluorescent compound?   omer.kabil
• primer design   p.molloy
• Cultivation of anaerobic bacteria   pclee at KAIST.AC.KR
• PAGE question   pjf
• I've had it with jtsr-stock@jtsr-stock.com!   r_m_apson at bigfoot.com
• How to get dimers, trimers.. by PCR of gene family   soren k rasmussen
• peptide database   soren k rasmussen
• Mobility shift problem...   rebecca
• Throw gloves after handling EtBr?   rebecca
• semi-specific PCR   rebecca
• Ubiquitous primers   rebecca
• Urgent help for plasmid purification   rebecca
• Purification of end labelled oligo probes?   rebecca
• gene identification troubles   rebecca
• PLEASE HELP...RNA/protein binding   rebecca
• Q: Transfection control plasmid   rebecca
• G protein beta-gamma subunits   rohr
• cells BRL(DE3)pLysS   ruben.varela-calvino at kcl.ac.uk
• Kras positive control for IHC   s.pritchard
• Kras positive control for LungIHC   s.pritchard
• For HELP: ligation of BIG inserts(ת¼Ä)   samy.bbs at DNS.CST.SH.CN
• PCR probe labeling   sarah_petersen at yahoo.com
• Ad: 8.9 cents/min Long Distance & 800 Service-NO Fees-NO Minimums!!   sender
• About firefly luciferase. Help!   shangzd at public.bta.net.cn
• Who can help me about pJLA503?   shangzd at public.bta.net.cn
• commercial luciferase vectors?   shangzd at public.bta.net.cn
• Ligation and electroporation   shira at LEONARDO.LS.HUJI.AC.IL
• Your three steps   shr at PROFFER.COM
• dynazyme ext   steven
• Growing BAC cultures successfuly   suglueck at indiana.edu
• RNA extraction from pancreas   theodorn at medlib.georgetown.edu
• IMS-PCR   tom
• genomic library   tom
• Selection for large inserts in plasmids?   clive tregaskes
• Rosmarinic acid   tsir
• SOD   tsir
• Q: 3'UTR sequence programs   bradley turner
• Mutagenesis   bradley turner
• 17-BETA-HYDROXYSTEROID-OXIDOREDUCTASE   unixon_31 at hotmail.com
• murine T cell hybridomas   vaumieu
• extra G in 5' RACE   vilimf01 at mcrcr6.med.nyu.edu
• mouse/rat synteny   wai
• (none)   walshrr at EMAIL.UC.EDU
• Gel Shift Question   walshrr at EMAIL.UC.EDU
• pCMV1Flag   zhi_xue wang
• Sequencing gels -bubbles   weazel at blarg.net
• Tons of FREE Stuff!   webmaster at AOL.COM
• Tons of FREE Stuff!   webmaster at AOL.COM
• cell fractionation method?   wgschech at med.uni-tuebingen.de
• S1/Mung Bean   wtd3 at columbia.edu
• Air gel dry   yangt at CUHK.EDU.HK
• DDRT-PCR   yjiang
• Ask for help on RT-PCR   yww
• (none)   yww
• sensitivity of methylene blue staining ?   z

Last message date: Tue Mar 31 23:13:06 EST 1998
Archived on: Sun Mar 6 21:40:46 EST 2005

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