Eluting DNA from agarose gels
Virginia Wotring, PhD
wotring at nrc.uab.edu
Fri May 15 14:04:15 EST 1998
I'd like to return to the gel-purification-may-inhibit-ligations thread
for an opinion poll.
When gel purifying, what methods do you use for eluting DNA from an
agarose gel to
get the best ligations? Kits with columns/resins? Electroelution?
membranes? Do you find that the size of the DNA fragment (i.e > 6kb,
or < 500bp)
you are isolating makes a difference in recovery with certain methods?
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