Eluting DNA from agarose gels

Virginia Wotring, PhD wotring at nrc.uab.edu
Fri May 15 14:04:15 EST 1998


I'd like to return to the gel-purification-may-inhibit-ligations thread
for an opinion poll.
When gel purifying, what methods do you use for eluting DNA from an
agarose gel to
get the best ligations?  Kits with columns/resins?  Electroelution?
DEAE-cellulose
membranes?  Do you find that the size of the DNA fragment  (i.e > 6kb,
or < 500bp)
you are isolating makes a difference in recovery with certain methods?

Just curious,

Ginger




More information about the Methods mailing list