Cannot resolubilize genomic DNA Gunnaporn Veeracha
gkidd at aptagen.com
Mon Nov 2 12:13:46 EST 1998
I had this problem once, when I first tried isolating genomic DNA. It was impossible to re-dissolve. As a final act of mad vengence, I even tried soniccation, but that failed as well. I suspect that unfortunately you'll have to toss it and start over. As
Reinhard said above, in the future don't over-dry it. It is possible to overdry even without lyophilization; air drying can do it too. Hope this helps.
Geoffrey Kidd, Ph.D.
Technical Services Manager
Gunnaporn - Veerachato wrote:
> Dear all
> I have extracted genomic DNA from white blood cells by using DNAzol BD kit (Gibco BRL). However, I cannot resolubilize my genomic DNA with 8mM NaOH although keeping at 4C for overnight or 37C for half a day. Could you please help me solve the problem?
> Thanks in advance.
> Gunnaporn Veerachato
> vgunnapo at mailcity.com
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