In vitro generation of multimers of gene which has BglII sites: HELP!

Yang Sheng shengyang at USA.NET
Tue Nov 3 01:12:01 EST 1998

Hi, all:

I want to generate multimers of my gene in vitro by clone it into 
pAO815. But there are 2 BglII sites within my gene. So, I can not 
use BglII and BamHI to cut my gene and ligate the fragments into 
multimers. Then, what can I do?

Thanks for any advice


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