low stringency hybridization

Guo-Yun Yu gyy at tc.umn.edu
Mon Nov 2 14:28:31 EST 1998


Does anybody have a protocal for low stringency hybridization screening
of cDNA library?  I need a protocal that I can easily change the
striengency.  I have a dna probe which is about 40% identity with the
target cDNA clone, at protein level.  I think I need a really low low
stringency hybirdization, and I have to do lots of test using positive
control.
Could you suggust to me some protocals?
Thanks!






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