In vitro generation of multimers of gene which has BglII sites: HELP!
Richard Sucgang, PhD
richard at pleaseREMOVEmonarchy.com
Tue Nov 3 04:11:01 EST 1998
In article <199811030546.NAA23925 at mail.shcnc.ac.cn>, shengyang at usa.net wrote:
>I want to generate multimers of my gene in vitro by clone it into
>pAO815. But there are 2 BglII sites within my gene. So, I can not
>use BglII and BamHI to cut my gene and ligate the fragments into
>multimers. Then, what can I do?
How about using XhoI and SalI instead?
This was a technique used to make multimers of the lacO site. Try looking
for Andrew Belmont's paper on it.
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