help about equilibrate phenol

Gerd gerdn at
Tue Nov 3 09:33:19 EST 1998

Bethune international peace hospital wrote:
> Hi
>  How to equilibrate phenol to a PH>7.8?
>     We have equilibrate phenol for one week with Tris.Cl(1M,PH8.0),
> but the PH of penol phase is still under 7.0  and much phenol lose.
>             thank you for help
> responds to:
>        genet at
Is it not the buffer pH that should be meassured? Think I remember
correct pH cannot be meassured directly in phenol.
Stop eq. when buffer pH stays at the pH of choice.

More information about the Methods mailing list