help about equilibrate phenol
gerdn at ibg.uit.no
Tue Nov 3 09:33:19 EST 1998
Bethune international peace hospital wrote:
> How to equilibrate phenol to a PH>7.8?
> We have equilibrate phenol for one week with Tris.Cl(1M,PH8.0),
> but the PH of penol phase is still under 7.0 and much phenol lose.
> thank you for help
> responds to:
> genet at public.sj.he.cn
Is it not the buffer pH that should be meassured? Think I remember
correct pH cannot be meassured directly in phenol.
Stop eq. when buffer pH stays at the pH of choice.
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