help about equilibrate phenol

David M. Eisenmann eisenman at umbc.edu
Tue Nov 3 09:19:16 EST 1998


In article <01bdfa3d$d1a14c80$LocalHost at ns.sj.he.cn>,
genet at PUBLIC.SJ.HE.CN ("Bethune international peace hospital") wrote:

> Hi
>  How to equilibrate phenol to a PH>7.8?
>     We have equilibrate phenol for one week with Tris.Cl(1M,PH8.0),
> but the PH of penol phase is still under 7.0  and much phenol lose.
>             thank you for help 
> responds to:
>        genet at public.sj.he.cn

If you mean you have left the *same* 1M Tris Ph 8.0 in there for one week,
that's not going to work.  Take off the aqueous phase and do repeated
equilibrations with fresh Tris each time.

Dave



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