help about equilibrate phenol
David M. Eisenmann
eisenman at umbc.edu
Tue Nov 3 09:19:16 EST 1998
In article <01bdfa3d$d1a14c80$LocalHost at ns.sj.he.cn>,
genet at PUBLIC.SJ.HE.CN ("Bethune international peace hospital") wrote:
> How to equilibrate phenol to a PH>7.8?
> We have equilibrate phenol for one week with Tris.Cl(1M,PH8.0),
> but the PH of penol phase is still under 7.0 and much phenol lose.
> thank you for help
> responds to:
> genet at public.sj.he.cn
If you mean you have left the *same* 1M Tris Ph 8.0 in there for one week,
that's not going to work. Take off the aqueous phase and do repeated
equilibrations with fresh Tris each time.
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