Retinoic acid and retinol solutions

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Tue Nov 3 09:25:41 EST 1998


On 3 Nov 1998 10:29:02 -0000, JOSE LUIS CARRASCO JIMENEZ
<jlcarrasco at ibmcp.upv.es> wrote:

>
>  Dear colleagues,
>
>  I would highly appreciate any information you may provide
>me with about solvents to use in the preparation of retinoic
>acid and all-trans retinol stock solutions and the right way
>to store those solutions.
>
>  Many thanks in advance
>
>  Best wishes,
>
>  Jose L. Carrasco
>  jlcarrasco at ibmcp.upv.es
Dear Jose
We rountinely prepare 10-2M retinoic acid in DMSO.
We store and further dilute it in wheaton-glass vials (important
especially for low concentrations, which could be absorbed by
hydrophobic plastic vials)
wrapped with Alu-foil, to keep the contents dark at -80°C. We use
reduced light conditions when applying the ret. acid. to tissue
cultures. We have no experience with retinol, but I think a similar
procedure should be approbiate for retinol, too.
hope this is of some help to you
martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
(remove .nospam before mailing)



More information about the Methods mailing list