help about equilibrate phenol
s3148487 at STUDENT.ANU.EDU.AU
Tue Nov 3 19:04:53 EST 1998
Wouldn't the presence of methanol inhibit PCR in some way? I thought I
heard somewhere that I did. Would a chloroform clean-up get rid of enough
>Gerd (gerdn at ibg.uit.no) wrote:
>: Bethune international peace hospital wrote:
>: > Hi
>: > How to equilibrate phenol to a PH>7.8?
>: > We have equilibrate phenol for one week with Tris.Cl(1M,PH8.0),
>: > but the PH of penol phase is still under 7.0 and much phenol lose.
>: > thank you for help
>: > responds to:
>: > genet at public.sj.he.cn
>: Is it not the buffer pH that should be meassured? Think I remember
>: correct pH cannot be meassured directly in phenol.
>: Stop eq. when buffer pH stays at the pH of choice.
>Actually there is a method where you add the phenol to methanol that
>works (allows measurement with a pH probe). I can't recall exactly
>what the proportion was though.
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