Cloning 11 Kb PCR Products
aeg17 at campfire.rrz.uni-koeln.de
Wed Nov 4 10:38:30 EST 1998
A friend of mine cloned an 18 kb fragment with the Boehringer Mannheim
"PCR cloning kit (blunt ends)".
Best regards, Daniel Schlieper
Muhammad_Lodhi at axyspharm.com ("Muhammad Lodhi") writes:
> I am trying to clone an 11 Kb PCR product, after a long time. I am
> wondering if someone could recommend me a good cloning vector or
> commercial kit. I need to clone lots of products. I was told that
> Invitrogen's TOPO XL PCR Cloning Kit works well. Any suggestions
> will be highly appreciated.
> Best regards
> Muhammad A. Lodhi
> Axys Pharmaceuticals, Inc.
Daniel Schlieper Institut fuer Biochemie
Zuelpicher Strasse 47
Daniel.Schlieper at Uni-Koeln.De Universitaet zu Koeln
Tel.: +49 221 470 6443 D-50674 Koeln, FRG
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