Cloning 11 Kb PCR Products

[Daniel Schlieper]

Dear Muhammad,

A friend of mine cloned an 18 kb fragment with the Boehringer Mannheim
"PCR cloning kit (blunt ends)".

Best regards, Daniel Schlieper


Muhammad_Lodhi at axyspharm.com ("Muhammad Lodhi") writes:

> Hi 
> I am trying to clone an 11 Kb PCR product, after a long time.  I am
> wondering if someone could recommend me a good cloning vector or
> commercial kit.  I need to clone lots of products.  I was told that
> Invitrogen's TOPO XL PCR Cloning Kit works well.  Any suggestions
> will be highly appreciated.  
> Best regards 
> Muhammad A. Lodhi 
> Axys Pharmaceuticals, Inc.

-- 
Daniel Schlieper                    Institut fuer Biochemie
                                    Zuelpicher Strasse 47
Daniel.Schlieper at Uni-Koeln.De       Universitaet zu Koeln 
Tel.: +49 221 470 6443              D-50674 Koeln, FRG