HELP! - cloning problem

MSRA MSRA at novo.dk
Wed Nov 4 19:40:14 EST 1998


     Dear Daniel,
     
     Thank you for the following comment.  Yes, I have successfully cloned 
     the whole coding region of the gene in a T-vector.  It went just fine. 
      I also cloned the gene from a different bacterial species, which is 
     pretty similar to what I am going to clone, successfully into pBAD.  
     So my guess is it may not be at least very toxic...?
     
     Mari
     
     
     ------------------------------
     Hello Mari,
     
     
     sounds like you try to clone a toxic gene. Does it work to clone it
     into a completely other vector? Like a non-gene region of pB322, or do
     you need it in pBAD or pMAL?
     
     
     Regards, Daniel



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