searching for HLA negative cell line
jladasky at pmgm.Stanford.EDU
Thu Nov 5 21:51:48 EST 1998
In article <71tkl6$e7d at panix3.panix.com>, Ian A. York <iayork at panix.com> wrote:
>In article <firstname.lastname@example.org>,
>alex dobrovic <adobrovic at medicine.adelaide.edu.au> wrote:
>>Daudi is class 1 negative because it lacks B2 microglobulin. It may still
>>express class 1 mRNAs though I am not sure if anyone has looked at this.
>Daudi is *surface* negative for MHC class I (because it lacks b2m). It
>expresses the MHC class I proteins perfectly well, though they're retained
>in the ER; and if beta-2 m is expressed in the cell the surface expression
>of the MHC types is restored. Daudi has been haplotyped (it has an unusual
>type), but I can't remember by whom.
Daudi has been genotyped for HLA-A, -B, and -C. However I don't
know that the haplotypes have been examined. The genotype is discussed in:
Browning MJ, et al.
The HLA-A,B,C genotype of the class I negative cell line Daudi
reveals novel HLA-A and -B alleles.
Tissue Antigens. 1995 Mar;45(3):177-87.
Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw. - John Ladasky
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