searching for HLA negative cell line
jladasky at pmgm.Stanford.EDU
Thu Nov 5 21:47:38 EST 1998
Whoops! That was probably unclear. Yes, Daudi does express
class I *heavy chain* mRNA. Transfection of B2m into Daudi cells is
sufficient to restore the expression of HLA-A, -B, and -C. This is
discussed in the following articles:
Quillet A, et al.
Increased resistance to non-MHC-restricted cytotoxicity related
to HLA A, B expression. Direct demonstration using beta 2-micro-
globulin-transfected Daudi cells.
J Immunol. 1988 Jul 1;141(1):17-20.
Seong RH, et al.
Rescue of Daudi cell HLA expression by transfection of the mouse
beta 2-microglobulin gene.
J Exp Med. 1988 Feb 1;167(2):288-99.
The articles that I quote below refer to *B2m* mRNA, which is
also expressed. As I recall, one of two B2m loci is deleted entirely.
The remaining expressed B2m mRNA has a premature stop codon.
In article <71tjmk$cn5 at pmgm.Stanford.EDU>,
John Ladasky <jladasky at pmgm.Stanford.EDU> wrote:
>In article <firstname.lastname@example.org>,
>alex dobrovic <adobrovic at medicine.adelaide.edu.au> wrote:
>>Daudi is class 1 negative because it lacks B2 microglobulin. It may still
>>express class 1 mRNAs though I am not sure if anyone has looked at this.
> Someone has, and it does, from one of the two damaged B2m loci.
> de Preval C, et al.
> The absence of beta 2-microglobulin in Daudi cells: active gene but
> inactive messenger RNA.
> Immunogenetics. 1983;17(2):133-40.
> Rosa F, et al.
> Presence of an abnormal beta 2-microglobulin mRNA in Daudi cells:
> induction by interferon.
> Immunogenetics. 1983;17(2):125-31.
Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw. - John Ladasky
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