searching for HLA negative cell line

John Ladasky jladasky at pmgm.Stanford.EDU
Thu Nov 5 21:47:38 EST 1998

	Whoops!  That was probably unclear.  Yes, Daudi does express 
class I *heavy chain* mRNA.  Transfection of B2m into Daudi cells is
sufficient to restore the expression of HLA-A, -B, and -C.  This is
discussed in the following articles:

	Quillet A, et al.
	Increased resistance to non-MHC-restricted cytotoxicity related
	to HLA A, B expression. Direct demonstration using beta 2-micro-
	globulin-transfected Daudi cells. 
	J Immunol. 1988 Jul 1;141(1):17-20. 

	Seong RH, et al.
	Rescue of Daudi cell HLA expression by transfection of the mouse
	beta 2-microglobulin gene. 
	J Exp Med. 1988 Feb 1;167(2):288-99. 

	The articles that I quote below refer to *B2m* mRNA, which is
also expressed.  As I recall, one of two B2m loci is deleted entirely.
The remaining expressed B2m mRNA has a premature stop codon.

In article <71tjmk$cn5 at pmgm.Stanford.EDU>,
John Ladasky <jladasky at pmgm.Stanford.EDU> wrote:
>In article <l03130302b267fa8ba8d7@[]>,
>alex dobrovic <adobrovic at> wrote:
>>Daudi is class 1 negative because it lacks B2 microglobulin. It may still
>>express class 1 mRNAs though I am not sure if anyone has looked at this.
>	Someone has, and it does, from one of the two damaged B2m loci.
>	de Preval C, et al.
>	The absence of beta 2-microglobulin in Daudi cells: active gene but
>	inactive messenger RNA. 
>	Immunogenetics. 1983;17(2):133-40. 
>	Rosa F, et al.
>	Presence of an abnormal beta 2-microglobulin mRNA in Daudi cells:
>	induction by interferon. 
>	Immunogenetics. 1983;17(2):125-31. 

Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw.					- John Ladasky

More information about the Methods mailing list