searching for HLA negative cell line

alex dobrovic adobrovic at medicine.adelaide.edu.au
Thu Nov 5 20:04:34 EST 1998


Daudi is class 1 negative because it lacks B2 microglobulin. It may still
express class 1 mRNAs though I am not sure if anyone has looked at this.

>David L. Haviland, Ph.D. <dhavilan at IMM2.IMM.UTH.TMC.EDU> wrote:
>>
>>Going strictly from memory,  Daudi (B cell) and K562 (erythroleukemic) come
>>to mind as Class-I negative.  I also have it in my advisors handwriting
>>(from *many* moons ago) that the B cell line Raji is also Class-I negative.
>
>K562 are HLA negative, Raji are not.  You need to be careful with studies
>on K562, because they upregulate their MHC when treated with interferon
>(and various other things).  I don't know whether they have non-classical
>class I.
>
>Ian
>--
>Ian York

Alexander Dobrovic, Ph.D.
Chief Medical Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia

Tel 61-8-8222 7676
Fax 61-8-8222 6046
adobrovi at medicine.adelaide.edu.au





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