searching for HLA negative cell line
adobrovic at medicine.adelaide.edu.au
Thu Nov 5 20:04:34 EST 1998
Daudi is class 1 negative because it lacks B2 microglobulin. It may still
express class 1 mRNAs though I am not sure if anyone has looked at this.
>David L. Haviland, Ph.D. <dhavilan at IMM2.IMM.UTH.TMC.EDU> wrote:
>>Going strictly from memory, Daudi (B cell) and K562 (erythroleukemic) come
>>to mind as Class-I negative. I also have it in my advisors handwriting
>>(from *many* moons ago) that the B cell line Raji is also Class-I negative.
>K562 are HLA negative, Raji are not. You need to be careful with studies
>on K562, because they upregulate their MHC when treated with interferon
>(and various other things). I don't know whether they have non-classical
Alexander Dobrovic, Ph.D.
Chief Medical Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia
Tel 61-8-8222 7676
Fax 61-8-8222 6046
adobrovi at medicine.adelaide.edu.au
More information about the Methods