Cannot resolubilize genomic DNA Gunnaporn Veeracha

Edith Duerbaum Edith.Duerbaum at post.rwth-aachen.de
Thu Nov 5 10:46:53 EST 1998


Reinhard Hoffmann schrieb in Nachricht <363DEA24.40EC at mail.bii.ch>...
>You probably overdried the pellets. Have you dried it in a speedvac? be
>careful, dry shorter. Good luck.


Dear Gunnaporn,


I have problems with resolubilisation (? is this the word?)  only when the
DNA isn´t very clean.
Usually it works fine in TE with gentle shaking at 56°C: takes several
minutes, an hour at the  longest....

Good luck,


    Edith






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