adeniine detection

Young, Ronald J. rxyoung at CBDCOM-EMH1.APGEA.ARMY.MIL
Thu Nov 5 16:32:25 EST 1998


Does anyone have a sensitive and mild method for the detection of
adenine released from an enzymatic reaction. It is possible to convert
adenine to the fluorescent ethenoadenine by reacting with bromo- or
chloroacetaldehyde, but unfortunately this requires heating at about 85C
for sometime. Please reply directly as I am not sure I am a member of
the list yet.

Ron



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