mwcrepeau at ucdavis.edu (Marc
Crepeau) wrote:
>Has anyone had any luck purifying
their own Klentaq from an E. coli
>clone?
Whoa. Before you implerent all
those plans, perhaps what you have
not a poor extraction, but a loss of
activity due to inadequate DTT. I
use 10 mM personally to ensure a
well protected active site upon
removal of DNA by the column.