purification of Klentaq

Darryl L. Clark dlclark at ksu.edu
Fri Nov 6 00:41:06 EST 1998


mwcrepeau at ucdavis.edu (Marc 
Crepeau) wrote:
>Has anyone had any luck purifying 
their own Klentaq from an E. coli
>clone?

      Whoa.  Before you implerent all 
those plans, perhaps what you have 
not a poor extraction, but a loss of 
activity due to inadequate DTT.  I 
use 10 mM personally to ensure a 
well protected active site upon 
removal of DNA by the column.




More information about the Methods mailing list