Uneven growth of lambda phage

[luc]

make shure  pouring bottom AND top agar on a perfectly leveled table

luc

JOSE LUIS CARRASCO JIMENEZ wrote:

>   Dear colleagues,
>
>   I am having problems in plating a lambdaZap library on large
> plates. Phages only grow on a half-moon section of the plate,
> but much slower on the other half. Does any of you have
> any idea about what the reason may be? I am being careful
> to spread bottom and top agar horizontal.
>
>   Any suggestion will be most welcome and appreciated.
>
>   With best regards,
>
>   Jose
>
>   Jose Luis Carrasco
>   Instituto de Biologia Molecular y Celular de Plantas
>   Camino de Vera, s/n
>   46022 Valencia
>   Spain
>   Email: jlcarrasco at ibmcp.upv.es