Just digest your sample with ssDNA exonuclease such as Exo I. Simple treatment, try it! On Fri, 6 Nov 1998, Fraser Dr N J wrote: > Probably a simple question, but can't think of the answer at the moment. > How do I remove a 5' overhang ? I want to destroy an NcoI site (CCATGG) > to remove the ATG so simple filling in won't do. > Cheers - Neil > >