Removal of 5' overhang

Mr K Dai kdai at hgmp.mrc.ac.uk
Mon Nov 9 06:49:54 EST 1998


Just digest your sample with ssDNA exonuclease such as Exo I. Simple
treatment, try it!

On Fri, 6 Nov 1998, Fraser Dr N J wrote:

> Probably a simple question, but can't think of the answer at the moment.
> How do I remove a 5' overhang ? I want to destroy an NcoI site (CCATGG)
> to remove the ATG so simple filling in won't do.
> Cheers - Neil
> 
> 




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