Removal of 5' overhang

Keith Rand Keith.Rand at molsci.csiro.au
Tue Nov 10 01:48:06 EST 1998


In article <Pine.GSO.3.94.981109114835.1349B-100000 at tin>, Mr K Dai
<kdai at hgmp.mrc.ac.uk> wrote:

> Just digest your sample with ssDNA exonuclease such as Exo I. Simple
> treatment, try it!
> 
> On Fri, 6 Nov 1998, Fraser Dr N J wrote:
> 
> > Probably a simple question, but can't think of the answer at the moment.
> > How do I remove a 5' overhang ? I want to destroy an NcoI site (CCATGG)
> > to remove the ATG so simple filling in won't do.
> > Cheers - Neil
> > 
> > 

Isn't Exo I a 3'-5' exonuclease?

-- 
Keith Rand,  Sydney Australia



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