DNA cleanup - RNAse free

Jens Mollerup jmollerup at aki.ku.dk
Mon Nov 9 08:02:41 EST 1998


Hi Netters,

I need to remove RNA contamination of lambda DNA samples. RNAses are not
to be used since we will use cRNA (in vitro transkription) later in the
procedure. Off course we can run an agarose gel and purify from that,
but there might be an easier way. We have tried to precipitate RNA by
MgCl2 or LiCl2 - it doesnt work! Does anyone have an idea.

Thanks in advance
M.Sc. Jens Mollerup




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