DNA cleanup - RNAse free

Andrew Warkman awarkman at julian.uwo.ca
Mon Nov 9 09:30:46 EST 1998


In article <3646E7F0.8FE5FC00 at aki.ku.dk>, Jens Mollerup <jmollerup at aki.ku.dk> wrote:
>Hi Netters,
>
>I need to remove RNA contamination of lambda DNA samples. RNAses are not
>to be used since we will use cRNA (in vitro transkription) later in the
>procedure. Off course we can run an agarose gel and purify from that,
>but there might be an easier way. We have tried to precipitate RNA by
>MgCl2 or LiCl2 - it doesnt work! Does anyone have an idea.
>
(1) RNase A digestion 

(2) Proteinase K digestion

(3) phenol:chloroform extractions

Works great for me in preparing RNase free plasmid templates for RNA probe 
synthesis. Hope this helps...

Andy



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