DNA cleanup - RNAse free
awarkman at julian.uwo.ca
Mon Nov 9 09:30:46 EST 1998
In article <3646E7F0.8FE5FC00 at aki.ku.dk>, Jens Mollerup <jmollerup at aki.ku.dk> wrote:
>I need to remove RNA contamination of lambda DNA samples. RNAses are not
>to be used since we will use cRNA (in vitro transkription) later in the
>procedure. Off course we can run an agarose gel and purify from that,
>but there might be an easier way. We have tried to precipitate RNA by
>MgCl2 or LiCl2 - it doesnt work! Does anyone have an idea.
(1) RNase A digestion
(2) Proteinase K digestion
(3) phenol:chloroform extractions
Works great for me in preparing RNase free plasmid templates for RNA probe
synthesis. Hope this helps...
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