Cetus thermocyclers

Weining, Song WeininS at prose.dpi.qld.gov.au
Tue Nov 10 03:23:29 EST 1998


> From:	Weining, Song 
> Sent:	Tuesday, 10 November 1998 17:30
> To:	'methods at net.bio.net'
> Subject:	Re: Cetus thermocyclers
> 
> just resending in case anyone missed earlier discussion
> 
> 
> I agree with Sandra. I've used ALL of PE's machines and MOST of the
> peltier ones by various makers. anything older than 9700 has the problem
> of uneven heating/cooling. You can prove it by a proper thermometer. A lot
> PCRs are not stringent with the temperature profiles so you can get away
> with poorly performed machines. The original design of PE's is wrong and
> they already switched to peltier for a while now. 
> I don't know why anyone still wants to buy a 2400. small in sample block
> but not in price, outdated software and the performance? enough comment
> already there. It's like comparing G3 with DOS if you try some late
> peltier ones. 
> I'm not surprised with Sandra's experience with PE. PE is not renowned for
> their service as they are not REALLY a biotech company. But I have to say
> I like their spectrophotometers.
> 
> Song Weining
> Leslie Research Centre
> 13 Holberton Street
> PO Box 2282,
> Toowoomba, QLD 4350
> Australia
> weinins at dpi.qld.gov.au
> 
> To: methods at net.bio.net <mailto:methods at net.bio.net>
> From: sandra kay cooper <scooper1 at students.uiuc.edu
> <mailto:scooper1 at students.uiuc.edu>>
> Subject: Re: Cetus thermocyclers
> Date: Wed, 4 Nov 1998 14:06:45 -0600
> To: luc <luc at sgi.celgen.kuleuven.ac.be
> <mailto:luc at sgi.celgen.kuleuven.ac.be>>
> In-Reply-To: <36408B3E.2A81A88A at sgi.celgen.kuleuven.ac.be>
> Dr Black,
> I would strongly recommend AGAINST purchasing a PE2400. I just
> spent the last year and a half trying to get a refund on our PE2400. We
> had to return our machine 2 times in 2 years, and one of the loaners from
> PE had to be returned for repairs (while our original machine was being
> repaired). I was informed by a thermal-cycler repairman (not associated
> with PE) that the coolant they used in designing the original machines is
> inferior, and over time will "gunk" up. Also, the gel that they use to
> equalize temperatures across the block with "stiffen" over time. On two
> different PE2400s, I set up IDENTICAL reactions in each of the 24 wells
> (all reagents were pooled prior to aliquotting). When I ran products out,
> it turned out that only 12 of the wells were usable. Some of the outside
> corners gave no amplification product whatsoever, and other outside wells
> gave minimal amplification. they might be OK for everyday cloning, but Ii
> was doing quantitative work. 
> This isn't even talking about the lack of service I received from
> perkin-elmer and the runaround when I told them their machine was inferior
> and I wanted a refund. 
> I emphatically recommend an MJ Research machine over Perkin elmer.
> Not only are their machines superior, their staff is very helpful. It is
> possible to get a demo machine from them. The price is comprable to the
> PE machines, and they have interchangable blocks, multiple blocks, etc.
> Sandra Cooper
> University of Illinois
> On Wed, 4 Nov 1998, luc wrote:
> > we have the PE2400 and 9600 both on liquid cooling and the perform well.
> > Peltier machines have only given my trouble thusfar. i stay away from
> > them.
> > 
> > luc
> > 
> > Michael Black, Ph.D. wrote:
> > 
> > > Hello everyone, another query about thermocyclers.
> > >
> > > I have quotes for a Perkin-Elmer 2400 and 9700, and am trying to
> > > justify the $4200 USD price difference. Does anyone have experience
> > > with these two machines? Aside from the interchangable block
> > > configuration on the 9700, is it really any better a machine than the
> > > 2400? Any inside info would be appreciated.
> > >
> > > Michael
> > >
> > > --
> > > Michael Black, Ph.D.
> > > Post-Doctoral Fellow
> > > Univ. Texas at Austin
> > > Marine Science Institute
> > > mblack at utmsi.utexas.edu <mailto:mblack at utmsi.utexas.edu>
> > >
> > > * opinions expressed are, as far *
> > > * as I know, just my own. *
> 



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