½Íĸ˫ÔÓ½»Çë½Ì(ת¼Ä)

SYSOP.bbs at DNS.CST.SH.CN SYSOP.bbs at DNS.CST.SH.CN
Tue Nov 10 21:57:30 EST 1998


·¢ÐÅÈË: rocksheng (¹öʯ), ÐÅÇø: Method
±ê  Ìâ: Re: ½Íĸ˫ÔÓ½»Çë½Ì
·¢ÐÅÕ¾: ÉúÃüÐþ»ú (Tue Nov 10 13:30:12 1998) , Õ¾ÄÚÐżþ

There are several points may be help to your question, though you want
kick me out.
1. have you ever check out the cell toxicity of the transformed plasid
2. the hybrid proteins are not stably expressed in the host cell
3. the fused DNA-BD or AD occludes the sites of interaction
4. the hybrid protein folds improperly
5. the hybrid protein cannot be localized to the yeast nucleus
6. for unknown reasons, protein-protein interaction was not detectable using
LexA two-hybrid system, but was detectable using GAL4-based system.
7. the last reason, some protein-protein interactions may not be detectable
using any type of two-hybrid system

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