Ian A. York
iayork at panix.com
Tue Nov 10 14:49:06 EST 1998
In article <36488C28.9E6309D1 at gengenp.rug.ac.be>,
Esbjorn Fiers <esfie at gengenp.rug.ac.be> wrote:
>Thanks for your reaction. I should have mentioned that it is quite a
>trickysubcloning, I think. It is with 3 fragments. That is one of the reasons
My opinion is that it's rarely a timesaver to do a three-fragment cloning.
You'd usually get your result faster with a series of subclones, each with
two fragments. Run your fragments through different plasmids if
necessary to get the appropriate fragments.
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
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