cloning problems

Ian A. York iayork at
Tue Nov 10 14:49:06 EST 1998

In article <36488C28.9E6309D1 at>,
Esbjorn Fiers  <esfie at> wrote:
>Thanks for your reaction. I should have mentioned that it is quite a
>trickysubcloning, I think. It is with 3 fragments. That is one of the reasons

My opinion is that it's rarely a timesaver to do a three-fragment cloning.
You'd usually get your result faster with a series of subclones, each with
two fragments.  Run your fragments through different plasmids if
necessary to get the appropriate fragments.

    Ian York   (iayork at  <>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England

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