totalRna Extraction from Salmonella

Audrey Caro acaro at email.unc.edu
Wed Nov 11 15:55:22 EST 1998


I had trouble to isolate large quatity of total RNA from Salmonella,
compared with E. coli. I have tried two kits, the one from Bio 101 (for
bacteria) and the one from Biotex (Rna Tack). I 've tried to extract
total RNA from 20ml of an overnight culture in TS broth. How can I be
sure that the lysis is correct? I tried once to add some lysosyme and
the result was not better.  Thanks for your suggestion.
Audrey CARO




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