histidine marker for CATIONIC PAGE

[Camile S Farah]

Can U help?

I am trying to run murine saliva and salivary gland extract on Cationic
Page, as developed by Baum and modified by MacKay. The histatins
<histidine rich proteins> that i am interested in are low molecular
weight (3-5 kd), positively charged proteins that run best on gels mage
of 15% acrylamide in acaetic acid/KOH, and buffers of acetic acid and
glycine. I do get bands on the gels and have worked out how to use the
system.
My question is: does anyone know of a marker I can use to determine the
molecular weights of the bands I'm getting on the gels.
thanks
C Farah
Oral Biology and Pathology
UQ, Australia
email: c.farah at mailbox.uq.edu.au