Antisense RNA plasmids

Sarah Roberts-Thomson S.Roberts-Thomson at PHARMACY.UQ.EDU.AU
Fri Nov 13 00:48:36 EST 1998


I would like to express an antisense RNA in cell culture and I am unsure
of what vectors I can use. Is it just a matter of using any vector used
in protein expression and presuming that the antisense orientation will
be transcribed but not translated. I was thinking of using one of the
tetracycline inducible plasmids from Clontech. Any advice would be much
 appreciated.

     Thanks

     Sarah Roberts-Thomson
     The University of Queensland




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