buffer gradient sequencing?

Thomas Nilsson thomas.nilsson at hks.se
Fri Nov 13 10:41:22 EST 1998


Hello,

I am looking for a detailed protocol describing how to run a DNA
sequencing gel with a buffer gradient generated by using higher ionic
strength buffer in the lower tank. A dejanews search gave some ideas, like
adding 1 M sodium acetate to the lower tank buffer. Are there other ways
of generating buffer gradients? I plan to Promegas silver sequncing kit;
can a buffer gradient interfere with the silver staining?

Sincerely yours,

Thomas Nilsson

thomas.nilsson at hks.se



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